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PathScan® RP Phospho-RSK1 (Ser380) Sandwich ELISA Kit #7965

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  • ELISA

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    Supporting Data

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The Rapid Protocol (RP) PathScan® RP Phospho-RSK1 (Ser380) Sandwich ELISA Kit from Cell Signaling Technology is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of RSK1 when phosphorylated at Ser380 in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the coated microwell plate forms a sandwich with RSK1 protein phosphorylated at Ser380 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of RSK1 protein phosphorylated at Ser380. Learn more about all of your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to the kit.

    Protocol

    Specificity / Sensitivity

    PathScan® RP Phospho-RSK1 (Ser380) Sandwich ELISA Kit #7965 detects endogenous levels of RSK1 protein when phosphorylated at Ser380. The kit sensitivity is shown in Figure 1. This kit detects proteins from indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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