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PathScan^® RP Phospho-Tau (Thr217) Chemiluminescent Sandwich ELISA Kit

PathScan^® RP Phospho-Tau (Thr217) Chemiluminescent Sandwich ELISA Kit #87749

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ELISA

Supporting Data

REACTIVITY

H M R

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

The rapid protocol (RP) PathScan^® RP Phospho-Tau (Thr217) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tau protein phosphorylated at Thr217 in a reduced assay time of 1.5 hours. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-tau (Thr217) in a single step. The plate is then extensively washed and chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-tau (Thr217). Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan^® RP Phospho-Tau (Thr217) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of tau protein phosphorylated at Thr217. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Rat

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Database Links

UniProt ID: P10636-8

Entrez-Gene Id: 4137

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PathScan® RP Phospho-Tau (Thr217) Chemiluminescent Sandwich ELISA Kit #87749