Render Target: STATIC
Render Timestamp: 2025-01-17T06:03:56.346Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-25 01:38:14.294
Product last modified at: 2025-01-01T09:03:45.665Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® RP Total DRP1 Sandwich ELISA Kit #77895

Filter:
  • ELISA
ELISA Image 1: PathScan® RP Total DRP1 Sandwich ELISA Kit
Figure 1. Treatment of HeLa cells with nocodazole stimulates phosphorylation of DRP1 at Ser616, but does not affect levels of total DRP1. The relationship between lysate protein concentration from untreated and nocodazole-treated HeLa cells and the absorbance at 450 nm using the PathScan® RP Total DRP1 Sandwich ELISA Kit #77895 is shown in the upper figure. The corresponding western blots using DRP1 antibody (left panel) and phospho-DRP1 (Ser616) antibody (right panel) are shown in the lower figure. HeLa cells were either left untreated or treated with nocodazole (100 ng/mL) for 17 hr, and then lysed.

To Purchase # 77895

Cat. # Size Qty. Price
77895C 1 Kit
96 assays
$641
77895V1 5 Kits
480 assays
$3,125
77895V2 10 Kits
960 assays
$6,090
77895V3 20 Kits
1920 assays
$11,859
77895V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M R
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
DRP1 Rabbit mAb Coated Microwells #9085496 tests
DRP1 Rabbit Detection mAb #993751 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Total DRP1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of DRP1 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with DRP1 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of DRP1. Learn more about your ELISA kit options here.

​​​​​​​*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP Total DRP1 Sandwich ELISA Kit detects endogenous levels of DRP1 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Rat

Background

Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.