Render Target: STATIC
Render Timestamp: 2025-01-22T05:00:16.949Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-25 01:36:26.767
Product last modified at: 2025-01-01T09:07:10.797Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit #14119

Filter:
  • ELISA
PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit: Image 1
The relationship between the protein concentration of lysates from LNCaP cells and the absorbance at 450 nm as detected by the PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit is shown in the top figure. PSA/KLK3 protein is expressed in LNCaP cells but absent in other cell lines, as detected by western blotting using the PSA/KLK3 (D6B1) XP® Rabbit mAb as shown in the bottom figure.

To Purchase # 14119

Cat. # Size Qty. Price
14119C 1 Kit
96 assays
$641
14119V1 5 Kits
480 assays
$3,125
14119V2 10 Kits
960 assays
$6,090
14119V3 20 Kits
1920 assays
$11,859
14119V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
PSA/KLK3 Rabbit mAb Coated Microwells #6973396 tests
PSA/KLK3 Rabbit Detection mAb #268821 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol RP PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of PSA/KLK3 in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the coated microwell plate forms a sandwich with PSA/KLK3 in a single step. has been coated onto the microwells. After incubation with cell lysates, The plate is extensively washed and TMB reagent is added for signal development. The magnitude of the absorbance for this developed color is proportional to the quantity of total PSA/KLK3. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit detects endogenous levels of PSA/KLK3 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 amino-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.