PathScan® RP Total TFEB Sandwich ELISA Kit #36423
- ELISA
To Purchase # 36423
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 36423C | 1 Kit 96 assays | $641 | |
| 36423V1 | 5 Kits 480 assays | $3,125 | |
| 36423V2 | 10 Kits 960 assays | $6,090 | |
| 36423V3 | 20 Kits 1920 assays | $11,859 | |
| 36423V4 | 50 Kits 4800 assays | $28,845 |
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Product Specifications
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- Product Includes
- Related Products
| Product Includes | Volume | Solution Color |
|---|---|---|
| TFEB Rabbit mAb Coated Microwells #68473 | 96 tests | |
| TFEB Mouse Detection mAb #79965 | 1 ea | Red (Lyophilized) |
| HRP Diluent #13515 | 5.5 ml | Red |
| TMB Substrate #7004 | 11 ml | |
| STOP Solution #7002 | 11 ml | |
| Sealing Tape #54503 | 2 ea | |
| ELISA Wash Buffer (20X) #9801 | 25 ml | |
| Cell Lysis Buffer (10X) #9803 | 15 ml |
Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.
For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Total TFEB Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of TFEB protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with TFEB in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of TFEB protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Sardiello, M. et al. (2009) Science 325, 473-7.
- Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2.
- Settembre, C. et al. (2011) Science 332, 1429-33.
- David, R. (2011) Nat Rev Mol Cell Biol 12, 404.
- Cuervo, A.M. (2011) Science 332, 1392-3.
- Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58.
- Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81.
- Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8.
- Settembre, C. et al. (2012) EMBO J 31, 1095-108.
- Martina, J.A. et al. (2012) Autophagy 8, 903-14.
Limited Uses
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