Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo

PathScan® RP Total TFEB Sandwich ELISA Kit #36423

Filter:
  • ELISA
ELISA Image 1: PathScan® RP Total TFEB Sandwich ELISA Kit
Figure 1. HeLa cells express higher levels of TFEB protein compared with HCT 116 cells. The relationship between lysate protein concentration from HeLa and HCT 116 cells and the absorbance at 450 nm using the PathScan® RP Total TFEB Sandwich ELISA Kit #36423 is shown in the upper figure. The corresponding western blots using TFEB antibody (left panel) and β-Actin antibody (right panel) are shown in the lower figure.

To Purchase # 36423

Cat. # Size Qty. Price
36423C 1 Kit
96 assays
$641
36423V1 5 Kits
480 assays
$3,125
36423V2 10 Kits
960 assays
$6,090
36423V3 20 Kits
1920 assays
$11,859
36423V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Product Specifications

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
TFEB Rabbit mAb Coated Microwells #6847396 tests
TFEB Mouse Detection mAb #799651 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Total TFEB Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of TFEB protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with TFEB in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of TFEB protein. Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP Total TFEB Sandwich ELISA Kit detects endogenous levels of TFEB protein. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the upregulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in upregulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3).

Product #36423
Citations: 3

Add/Remove Filters
Sort by:
Publication Date Newest
Applications
Reactivity
Country
Showing 3 Citations
Journal:
Curr Issues Mol Biol
Author:
Jinglong Tang, et al.
Year:
2024
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
bioRxiv
Author:
Florentina Negoita, et al.
Year:
2024
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
bioRxiv
Author:
Yongchao Wang, et al.
Year:
2024
Applications:
Unspecified
Reactivity:
Unspecified
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.