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PDP - Template Name: Antibody Array Kit
PDP - Template ID: *******63c09b4
  1. Products /
  2. ELISA Kits /
  3. PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout)

PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout) #7949

This product is discontinued

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    Product Information

    Product Description

    The PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout) is a slide-based antibody array product founded upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 28 receptor tyrosine kinases and 11 important signaling nodes when phosphorylated at tyrosine or other residues. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two 8-pad slides, allowing the user to test up to 16 samples. Cell lysate is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated DyLight 680® is then used to visualize the bound detection antibody. A fluorescent image of the slide can then be captured with a digital imaging system and spot intensities quantified using array analysis software.

    Storage

    Kit should be stored at 4°C with the exception of Lysis Buffer, which is stored at –20°C (packaged separately).

    Specificity / Sensitivity

    Cell Signaling Technology's PathScan® RTK Signaling Antibody Array Kit detects the indicated RTKs and signaling nodes only when phosphorylated at tyrosine or specified residues (see Array Target Map). No significant crossreactivity has been observed between targets, with the exception of some crossreactivity of the FLT3 antibody with phosphorylated EphB3. In addition, Stat1 (Tyr701) and Stat3 (Tyr705) may be detected when phosphorylated at other tyrosine sites within the proteins. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see Figure 4). All capture antibodies have been validated for human targets. Although this kit has not been tested with mouse lysates, it is expected than many capture antibodies will crossreact in murine systems.

    Species Reactivity:

    Human

    Background

    Receptor Tyrosine Kinases (RTKs) are a family of cell surface receptors that signal primarily through tyrosine phosphorylation events (1). RTKs trigger a wide range of downstream signaling cascades, including the PI3K/Akt, MAPK and Jak/Stat pathways. These pathways control basic cellular functions such as division, growth, metabolism, differentiation, migration and survival. Dysregulation of RTK signaling has been implicated in a large number of cancers (2), making RTKs popular targets for pharmaceutical intervention.

    Database Links

    UniProt ID: P21709 , P40763 , P27361 , P29317 , P16234 , P35568 , P54753 , Q06418 , P04629 , P21860 , P31751 , Q9Y243 , P43403 , P07947 , P07948 , Q02763 , P30530 , P07333 , Q9UM73 , P62753 , P22455 , P28482 , P06239 , P00533 , P29320 , P31749 , P08581 , P11362 , P04626 , P10721 , P06213 , P54760 , P08631 , P07949 , P42224 , P08069 , Q16620 , P12931 , Q04912 , P35968 , P06241 , P00519


    Entrez-Gene Id: 2041 , 6774 , 5595 , 1969 , 5156 , 3667 , 2049 , 7301 , 4914 , 2065 , 208 , 10000 , 7535 , 7525 , 4067 , 7010 , 558 , 1436 , 238 , 6194 , 2264 , 5594 , 3932 , 1956 , 2042 , 207 , 4233 , 2260 , 2064 , 3815 , 3643 , 2050 , 3055 , 5979 , 6772 , 3480 , 4915 , 6714 , 4486 , 3791 , 2534 , 25


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.