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PDP - Template Name: ELISA Kit
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  1. Products /
  2. ELISA Kits /
  3. SARS-CoV-2 Spike Protein Serological IgM ELISA Kit

SARS-CoV-2 Spike Protein Serological IgM ELISA Kit #37322

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  • ELISA
ELISA Image 1: SARS-CoV-2 Spike Protein Serological IgM ELISA Kit
Patient Testing: Patient samples were tested using the SARS-CoV-2 Spike Protein Serological IgM ELISA Kit #37322. Serum or plasma was obtained from donors who had been diagnosed with SARS-CoV-2 (diagnosed positive n=49*) or from presumed uninfected donors collected prior to the SARS-CoV-2 outbreak (presumed negative n=86). Samples were heat-inactivated (56°C for 30 min) and diluted 1:400 prior to running the assay, as described in the protocol. Samples were considered positive, negative, or inconclusive based on the criteria described in the “Data Analysis” section of the protocol for the kit. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated from these data.

*Positive reference samples were from patient donors with a positive SARS-CoV-2 diagnosis. However, a positive SARS-CoV-2 diagnosis will not always correlate with a positive response in the IgM Serological ELISA as differences in disease severity and timing of sample collection relative to disease onset will affect presence and abundance of IgG, IgA, and IgM in the reference sample.

Note: We are continuing to test more samples as available. For the most up-to-date set of data, always refer to the product page for #37322 on the website.

1/4

To Purchase # 37322

Cat. # Size Qty. Price
37322C 1 Kit
96 assays
$730
37322V1 5 Kits
480 assays
$3,559
37322V2 10 Kits
960 assays
$6,935
37322V3 20 Kits
1920 assays
$13,505
37322V4 50 Kits
4800 assays
$32,850

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Spike Protein Coated Microwells II #8745596 tests
Anti-Human IgM, HRP-linked Antibody (ELISA Formulated) #157941 eaRed (Lyophilized)
Sample Diluent A #7163725 ml
HRP Diluent #1351511 mlRed
ELISA Wash Buffer (20X) #980125 ml
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Kit #37322 Positive Control #604971 ea
ELISA Kit #37322 Negative Control #338071 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The SARS-CoV-2 Spike Protein Serological IgM ELISA Kit is a solid-phase ELISA that detects binding of human IgM to full-length ectodomain SARS-CoV-2 spike trimeric protein (S-protein). Trimeric spike protein has been coated onto microwells. After incubation with sample, human IgM specific for trimeric spike protein is captured on the plate. The wells are then washed to remove unbound material. Anti-Human IgM, HRP-linked antibody is then used to recognize the bound IgM. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of IgM specific for spike protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The SARS-CoV-2 Spike Protein Serological IgM ELISA Kit detects endogenous levels of human IgM directed to full-length ectodomain SARS-CoV-2 spike trimeric protein (S-protein).

Species Reactivity:

Human

Background

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9).

Database Links

UniProt ID: P0DTC2


Entrez-Gene Id: 43740568


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For Research Use Only. Not For Use In Diagnostic Procedures.
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