PathScan® Tau (GT-38) Sandwich ELISA Kit #44712
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Alternative splicing of exon 10 results in the expression of two groups of tau: three-repeat and four-repeat tau. Isoforms 2, 4, and 5 express three microtubule binding repeat domains (Tau 3R), while isoforms 6, 7, and 8 express four microtubule binding repeat domains (Tau 4R) (4). Expression of Tau 3R and Tau 4R in cells can be different in mild or pathological conditions. For example, Tau 3R is preferentially expressed in Pick's disease (PiD) and corticobasal degeneration (CBD), while Tau 3R and Tau 4R are equally expressed in AD (5,6). The repeat-dependent tau has a different pattern of phosphorylation in different diseases, and also has the ability and patterns of aggregation (7-9). These varying patterns of aggregation result in disease-specific conformational structures, including hyperphosphorylated straight filaments (SFs) and PHFs in AD compared to SFs and twisted filaments in both PiD and CBD (10,11).
- Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
- Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
- Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
- Šimić, G. et al. (2016) Biomolecules 6, 6.
- Tuerde, D. et al. (2018) J Biol Chem 293, 1781-1793.
- Liu, C. and Götz, J. (2013) PLoS One 8, e84849.
- Weismiller, H.A. et al. (2018) J Biol Chem 293, 17336-17348.
- Goedert, M. et al. (2018) Cold Spring Harb Symp Quant Biol 83, 163-171.
- Kraus, A. et al. (2019) Acta Neuropathol 137, 585-598.
- Gibbons, G.S. et al. (2018) J Neuropathol Exp Neurol 77, 216-228.
- Henderson, M.X. et al. (2019) Acta Neuropathol Commun 7, 183.
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