Render Target: STATIC
Render Timestamp: 2025-01-21T12:45:47.937Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-25 01:36:56.472
Product last modified at: 2025-01-01T09:04:55.038Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® Total GFP Sandwich ELISA Kit #7878

Filter:
  • ELISA
ELISA Image 1: PathScan® Total GFP Sandwich ELISA Kit
Figure 1. Transfected GFP is detected by PathScan® Total GFP Sandwich ELISA Kit #7878. Lysates were prepared from control and GFP-transfected HEK293 cells and analyzed for GFP by PathScan® Total GFP Sandwich ELISA Kit #7878 or by Western blotting. Absorbance readings at 450 nm are shown in the top figure while the corresponding Western blot using GFP Antibody #2555 is shown in the bottom figure.

To Purchase # 7878

Cat. # Size Qty. Price Ships
7878C 1 Kit
96 assays
$641
7878V1 5 Kits
480 assays
$3,125
7878V2 10 Kits
960 assays
$6,090
7878V3 20 Kits
1920 assays
$11,859
7878V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY All
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
GFP Rabbit Antibody Coated Microwells #1060596 tests
GFP Mouse Detection mAb #142371 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total GFP Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects exogenously expressed GFP. This kit is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency. A GFP rabbit antibody has been coated onto the microwells. After incubation with cell lysates, GFP is captured by the coated antibody. Following extensive washing, a GFP mouse detection antibody is added to detect the captured GFP. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. The HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

CST's PathScan® Total GFP Sandwich ELISA Kit #7878 detects GFP when exogenously expressed in cells. As shown in Figure 1, this kit readily detects GFP in transfected, but not in untransfected HEK293 cell lysate. This kit was developed for use with transfected full-length GFP and may not detect GFP fusion proteins. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

All Species Expected

Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea. GFP maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.