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PathScan® Total HDAC4 Sandwich ELISA Kit #53804

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  • ELISA
ELISA Image 1: PathScan® Total HDAC4 Sandwich ELISA Kit
Figure 1. Detection of HDAC4 protein in the various cells is examined by using the PathScan® Total HDAC4 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using HDAC4 (D15C3) Rabbit mAb #7628 is shown in the bottom figure.

To Purchase # 53804

Cat. # Size Qty. Price Ships
53804C 1 Kit
96 assays
$641
53804V1 5 Kits
480 assays
$3,125
53804V2 10 Kits
960 assays
$6,090
53804V3 20 Kits
1920 assays
$11,859
53804V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M Mk
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
HDAC4 Rabbit mAb Coated Microwells #1024396 tests
HDAC4 Mouse Detection mAb #711861 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total HDAC4 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of HDAC4. An HDAC4 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, HDAC4 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, an HDAC4 mouse detection antibody is added to detect the captured HDAC4 protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of HDAC4 protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Total HDAC4 Sandwich ELISA Kit detects endogenous levels of HDAC4 protein, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Monkey

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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PathScan is a registered trademark of Cell Signaling Technology, Inc.
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