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PathScan® Total HER4/ErbB4 Sandwich ELISA Kit #13010

Filter:
  • ELISA
ELISA Image 1: PathScan® Total HER4/ErbB4 Sandwich ELISA Kit
Figure 1: Treatment of T-47D cells with Human Neuregulin-1 (hNRG-1) #5218 stimulates tyrosine phosphorylation of HER4/ErbB4 protein, as detected by PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit #13125, but does not affect the level of total HER4/ErbB4 detected by PathScan® Total HER4/ErbB4 Sandwich ELISA Kit #13010. The absorbance readings at 450 nm are shown in the top figure while corresponding western blots using HER4/ErbB4 (111B2) Rabbit mAb #4795 (left panel) and Phospho-HER4/ErbB4 (Tyr984) Antibody #3790 (right panel) are shown in the bottom figure.

To Purchase # 13010

Cat. # Size Qty. Price Ships
13010C 1 Kit
96 assays
$641 Feb 11
13010V1 5 Kits
480 assays
$3,125 Feb 11
13010V2 10 Kits
960 assays
$6,090 Feb 11
13010V3 20 Kits
1920 assays
$11,859 Feb 11
13010V4 50 Kits
4800 assays
$28,845 Feb 11

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
HER4/ErbB4 Rabbit mAb Coated Microwells #9719196 tests
HER4/ErbB4 Mouse Detection mAb #128201 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total HER4/ErbB4 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of HER4/ErbB4 protein. A HER4/ErbB4 rabbit mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-HER4/ErbB4 proteins are captured by the coated antibody. Following extensive washing, a HER4/ErbB4 mouse antibody is added to detect both the captured phospho- and nonphospho-HER4/ErbB4 proteins. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of HER4/ErbB4 protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Total HER4/ErbB4 Sandwich ELISA Kit #13010 detects endogenous levels of HER4/ErbB4 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
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