PathScan® Total SMAD2 Sandwich ELISA Kit #7244
- ELISA
Supporting Data
| REACTIVITY | H M Mi |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mi-Mink
Product Information
Product Description
CST's PathScan® Total SMAD2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total SMAD2 protein. A SMAD2 Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-SMAD2 proteins are captured by the coated antibody. Following extensive washing, SMAD2/3 Mouse mAb is added to detect both the captured phospho- and nonphospho-SMAD2 protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total SMAD2 protein.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Heldin, C.H. et al. (1997) Nature 390, 465-71.
- Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
- Derynck, R. et al. (1998) Cell 95, 737-40.
- Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Wrana, J.L. (2000) Sci STKE 2000, re1.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
- Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
Limited Uses
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