PathScan® Total Survivin Sandwich ELISA Kit #7169
- ELISA
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | H Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Description
CST's PathScan® Total Survivin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total survivin protein. A Survivin Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both phospho and nonphospho survivin proteins are captured by the coated antibody. Following extensive washing, Survivin Rabbit Detection Antibody is added to detect the captured survivin protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of total survivin protein.
*Antibodies in kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
- Li, F. et al. (1998) Nature 396, 580-584.
- Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
- O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
- Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
- Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.
Limited Uses
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