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Render Timestamp: 2024-12-26T12:17:53.654Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-04 20:01:11.765
Product last modified at: 2024-12-17T19:04:07.550Z
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PDP - Template Name: Protein Control Kit
PDP - Template ID: *******5c6809e
  1. Products /
  2. Experimental Controls /
  3. Elk-1 Control Proteins

Elk-1 Control Proteins #9183

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    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 15 µl of phosphorylated and nonphosphorylated Elk1 Control Proteins.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

    Product Description

    Nonphosphorylated Elk-1 Control Proteins: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer.
    Phosphorylated Elk-1 Control Proteins: Bacterially expressed Elk1 fusion protein phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer.
    MW (kDa) 40 (nonphosphorylated), 50 (phosphorylated)

    Background

    The ETS transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

    Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.

    Database Links

    UniProt ID: P19419


    Entrez-Gene Id: 2002


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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