HIF-1α/2α Control Cell Extracts #94790
- WB
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Product Description
HIF-1α/2α Control Cell Extracts (Hep G2 untreated): Total cell extracts from Hep G2 cells serve as a negative control. Supplied in SDS sample buffer.
HIF-1α/2α Control Cell Extracts (Hep G2 + CoCl2): Total cell extracts from Hep G2 cells treated with cobalt chloride (100 μM, 24 hr) serve as a positive control.
This lysate pair is produced as a control for western blotting of HIF-1α and HIF-2α proteins.
Background
HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).
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- Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11.
- Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9.
- Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004.
- Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82.
- Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62.
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