LC3 Control Cell Extracts #11972
- WB
Product Information
Product Usage Information
Boil for 3 minutes prior to use. Load 10 µl of untreated and chloroquine treated LC3 Control Cell Extracts per lane.
Storage
Store at –20°C, or at –80°C for long-term storage.
Product Description
LC3 Control Cell Extracts (HeLa +Chloroquine): Total cell extracts from HeLa cells treated with 50 μM chloroquine overnight serve as a positive control.
This lysate pair is produced as a control for western blotting of LC3A and LC3B. LC3C cannot be detected in these lysates.
Background
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
- Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
- Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
- Lang, T. et al. (1998) EMBO J. 17, 3597-607.
- Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
- He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
- Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
- Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
- Ichimura, Y. et al. (2000) Nature 408, 488-92.
- Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.
Limited Uses
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