Render Target: STATIC
Render Timestamp: 2024-11-21T12:42:19.190Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:21:44.105
Product last modified at: 2024-11-15T14:30:16.349Z
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PDP - Template Name: Cell Extracts
PDP - Template ID: *******b5396df

NF-κB Control Cell Extracts #9243

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    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 20 µl of untreated and hTNF-α treated NF-κB Control Cell Extracts per lane.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C or –80°C for long-term storage.

    Product Description

    NF-κB Control Cell Extracts (HeLa untreated): Total cell extracts from HeLa cells serve as a negative control. Supplied SDS Sample Buffer.

    NF-κB Control Cell Extracts (HeLa + hTNF-alpha): Total cell extracts from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 serve as a positive control. Supplied SDS Sample Buffer.

    Background

    Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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