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Render Timestamp: 2024-11-22T11:49:33.633Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:14:24.788
Product last modified at: 2024-06-27T13:36:09.967Z
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PDP - Template Name: Blocking Peptide
PDP - Template ID: *******6db2f4c

PTEN Blocking Peptide #1250

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    Product Information

    Product Usage Information

    Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5% glycerol and 1% DMSO. Store at –20°C.

    Product Description

    This peptide is used to block PTEN (D4.3) XP Rabbit mAb # 9188 reactivity.

    Quality Control

    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. This peptide is used to react with PTEN (D4.3) XP Rabbit mAb # 9188 in dot blot.

    Background

    PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
    Applications: Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols and for western immunoblot analysis.

    Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb # 9559 signal in immunohistochemistry.
    Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 datasheet.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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