CAR-T Cell (G4S Linker) Transduction Efficiency Flow Cytometry Panel #18839
Product Information
Protocol
Product Description
CD45 is a pan leukocyte marker. T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Monocytes are identified by the expression of CD14. CAR positive cells are identified by the expression of a G4S linker.
Product Usage Information
For individual product dilutions, refer to panel product datasheet or individual product pages.
Gating strategy for identifying viable, CAR positive T cell subsets: Gate on live cells using Propidium Iodide or 7-AAD as a histogram or scatterplot with forward scatter (FSC). Use a gate set on side scatter (SSC) vs. FSC to exclude cell debris. Singlets may be identified by setting a gate on FSC-H vs. FSC-A. Next, use a plot of CD45 vs. CD3 to identify CD45+ leukocytes, and CD3+ and CD3- subpopulations. Observe G4S Linker vs. CD3 expression and set a gate on CD3+G4S Linker+ cells, which are CAR+ T cells. Observe CD4 vs. CD8 expression within the gated population to identify CD4+ and CD8+ T cells that are CAR+. Similarly, observe G4S Linker vs. CD14 expression within the CD3- gate to identify contaminating monocytes.
Storage
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Limited Uses
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