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XML generation date: 2024-09-20 06:23:04.548
Product last modified at: 2024-12-17T18:51:09.102Z
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  1. Products /
  2. Flow Cytometry Kits & Reagents /
  3. Collagenase, Type 3

Collagenase, Type 3 #25970

    Product Information

    Product Usage Information

    Collagenase, Type 3 is soluble from 1-20 mg/ml with an optimal pH range of 6.3-7.5. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.

    Storage

    Collagenase, Type 3 is supplied as a lyophilized powder. This product is stable for 12 months when stored at 4ºC, protected from moisture. It is recommended to reconstitute as needed and to store solutions at a 1 mg/ml concentration at -20ºC or -80ºC. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Collagenase, Type 3 is isolated from Clostridium histolyticum and is lower in secondary proteolytic contaminant activity but has typical collagenase activity when compared to other collagenase types. Crude collagenase preparations contain a mixture of several different enzymes, including collagenases and proteases, that effectively break down intercellular matrices. The ratio of collagenolytic and proteolytic activities is an important part of tissue dissociation. Crude collagenase is inhibited by metal chelating agents, such as cysteine, EDTA, or o-phenanthroline, but not DFP. Calcium ions provide structural stability for enzyme activity (1). Collagenases are used at various concentrations for tissue dissociation, generally ranging from 0.1 to 5 mg/ml, and various time points depending on the desired affect (2-4). Several types of collagenases (Types 1, 2, 3, and 4) have been established based on the enzymatic profile.
    Activity Greater than or equal to 100 units per mg dry weight

    Unit Definition: One unit releases 1 μmol of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.

    Specificity / Sensitivity

    Collagenase, Type 3 is suggested for mammary primary cell isolation.

    Source / Purification

    Collagenase, Type 3 is produced by the bacterium Clostridium histolyticum and dialyzed prior to lyophilization.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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