Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel #78148
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Protocol
Product Description
T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Naive, effector, effector memory, and central memory T cell states can be distinguished by differential expression patterns of CD44, CD62L, and CD127/IL-7Rα.
Product Usage Information
Gating strategy for observing naive, effector, and memory T cell subsets:
If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Next, observe expression of CD44 vs. CD62L on either helper T cells or cytotoxic T cells. Naive T cells are CD44-CD62L+. Central memory T cells are CD44+CD62L+. Effector memory and effector cells are CD44+CD62L-. To distinguish effector memory and effector cells, observe expression of CD127/IL-7Rα on the CD44+CD62L- population. Effector memory cells are positive for expression of CD127/IL-7Rα. Effector cells are negative for expression of CD127/IL-7Rα. Naive and central memory T cells also express CD127/IL-7Rα.
Storage
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Specificity / Sensitivity
Species Reactivity:
Source / Purification
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