Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo

Phospho-SGK (Ser78) Antibody #3271

We recommend the following alternatives

Filter:
  • WB
Western Blotting Image 1: Phospho-SGK (Ser78) Antibody
Western blot analysis of extracts from HeLa cells untransfected (left lane) and transfected by HA-SGK (right lane), using Phospho-SGK (Ser78) Antibody (upper) or control SGK Antibody #3272 (lower). The phospho-specificity of the antibody was characterized by treating the membrane with or without calf intestinal alkaline phosphatase (CIP) after Western transfer.
This product is discontinued

Inquiry Info. # 3271

Please see our recommended alternatives.

Product Specifications

REACTIVITY H
SENSITIVITY Transfected Only
MW (kDa) 54 (Transfected only)
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-SGK (Ser78) Antibody detects transfected levels of SGK1 only when phosphorylated at serine 78. This antibody will not detect isoforms SGK2 or SGK3.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).
The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).

Alternate Names

Serum and glucocorticoid-inducible kinase

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.