5-Hydroxymethylcytosine (5-hmC) (HMC31) Mouse mAb #51660
- IF
- DB
To Purchase # 51660
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 51660T | 20 µl | $108 | |
| 51660S | 100 µl | $149 |
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- IF-Immunofluorescence
- DB-Dot Blot
Species Cross-Reactivity Key:
- All-All Species Expected
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| DNA Dot Blot | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TET protein-mediated cytosine hydroxymethylation was initially demonstrated in mouse brain and embryonic stem cells (5, 8). Since then this modification has been discovered in many tissues, with the highest levels found in the brain (9). While 5-fC and 5-caC appear to be short-lived intermediate species, there is mounting evidence showing that 5-hmC is a distinct epigenetic mark with various unique functions (10,11). The modified base itself is stable in vivo and interacts with various readers including MeCP2 (11,12). The global level of 5-hmC increases during brain development and 5-hmC is enriched at promoter regions and poised enhancers. Furthermore, there is an inverse correlation between levels of 5-hmC and histone H3K9 and H3K27 trimethylation, suggesting a role for 5-hmC in gene activation (12). Lower amounts of 5-hmC have been reported in various cancers including myeloid leukemia and melanoma (13,14).
- Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
- Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
- Okano, M. et al. (1999) Cell 99, 247-57.
- Li, E. et al. (1992) Cell 69, 915-26.
- Tahiliani, M. et al. (2009) Science 324, 930-5.
- He, Y.F. et al. (2011) Science 333, 1303-7.
- Ito, S. et al. (2011) Science 333, 1300-3.
- Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-30.
- Globisch, D. et al. (2010) PLoS One 5, e15367.
- Gao, Y. et al. (2013) Cell Stem Cell 12, 453-69.
- Mellén, M. et al. (2012) Cell 151, 1417-30.
- Wen, L. et al. (2014) Genome Biol 15, R49.
- Delhommeau, F. et al. (2009) N Engl J Med 360, 2289-301.
- Lian, C.G. et al. (2012) Cell 150, 1135-46.
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