Render Target: STATIC
Render Timestamp: 2024-11-12T10:31:21.014Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:28:34.764
Product last modified at: 2024-08-09T20:30:10.648Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

α-2-Macroglobulin Antibody #59002

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 163
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    α-2-Macroglobulin Antibody recognizes endogenous levels of total α-2-Macroglobulin protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro631 of human α-2-Macroglobulin protein.

    Background

    α-2-Macroglobulin (A2M) is a conserved tetrameric pan protease inhibitor found in many biological fluids, including plasma and cerebral spinal fluid. A2M can bind to most proteases, as well as growth factors, hormones, and cytokines. Clearance of A2M-bound proteins is mediated by low-density lipoprotein receptor-related protein 1 (LRP1/CD91) (1). Blood A2M levels decrease with age in humans, and this decrease may promote tumor development (2,3). In rodents, A2M modulates tumor cell adhesion, migration, and growth through inhibition of PI3K/Akt and SMAD pathways (3). Conversely, interaction with MMP2 may inhibit MMP2 activity in aged rat kidney, resulting in age-related accumulation of ECM and fibrosis (4). A2M may be a useful biomarker for liver fibrosis (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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