α-Actinin 4 (D7U5A) Rabbit mAb #15145
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The ubiquitously expressed α-actinin 4 (ACTN4) protein typically localizes to the cytoskeleton but extracellular stimuli will prompt nuclear translocation in some cells (4). Research studies suggest that ACTN4 can mediate cell signaling and regulate gene expression as a transcriptional coactivator (5,6). Increased expression of ACTN4 protein and amplification of the ACTN4 gene are seen in many cancers and correlates with poor prognosis and metastasis (7). Mutations in the corresponding ACTN4 gene are responsible for focal segmental glomerulosclerosis (FSGS1), a severe renal disorder characterized by reduced kidney function, proteinuria, and progressive kidney failure (8).
- Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11.
- Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66.
- Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21.
- Honda, K. et al. (1998) J Cell Biol 140, 1383-93.
- Aksenova, V. et al. (2013) Oncotarget 4, 362-72.
- Khurana, S. et al. (2011) J Biol Chem 286, 1850-9.
- Watabe, Y. et al. (2014) Cancer Med 3, 613-22.
- Kaplan, J.M. et al. (2000) Nat Genet 24, 251-6.
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