Render Target: STATIC
Render Timestamp: 2024-11-15T11:07:52.852Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:55:58.195
Product last modified at: 2024-11-05T19:15:10.189Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated) #56856

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H M R Hm
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:250

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier-free (BSA and azide free) version of this product see product #69319.

    Protocol

    Specificity / Sensitivity

    α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated) recognizes endogenous levels of total α-smooth muscle actin protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle protein.

    Background

    Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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