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α-Smooth Muscle Actin Antibody

α-Smooth Muscle Actin Antibody #14968

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	42
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

α-Smooth Muscle Actin Antibody recognizes endogenous levels of total α-smooth muscle actin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

Database Links

UniProt ID: P62736

Entrez-Gene Id: 59

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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