Render Target: STATIC
Render Timestamp: 2024-11-22T12:07:38.884Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:32.719
Product last modified at: 2024-11-07T12:15:25.596Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ABCA1 (E7X5G) Rabbit mAb #96292

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 254
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ABCA1 (E7X5G) Rabbit mAb recognizes endogenous levels of total ABCA1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1262 of human ABCA1 protein.

    Background

    ATP-binding cassette (ABC) proteins are membrane-residing transporters that transport substrates across the membrane in an ATP-dependent manner. ABC substrates subject to active transport across the membrane include ions, amino acids, lipids, and sterols (1). ATP-Binding cassette sub-family A member 1 (ABCA1) is a member of the ABC family and functions to regulate phospholipid and cholesterol homeostasis. ABCA1, like most ABC transporters, contains two transmembrane domain bundles composed of six membrane-spanning helices and two nucleotide-binding domains. ABCA1 and its closest homolog, ABCA7, are 12A class members of ABCs, and both proteins function to transport cholesterol and phospholipids in an apolipoprotein A (apoA)-dependent manner (2,3). ABCA1 is expressed in a variety of tissues (4). Loss of function mutations in the human ABCA1 gene are linked to Tangier disease, a disorder characterized by high density lipoprotein (HDL) deficiency and cholesterol ester buildup in macrophages, as well as increased risk of atherosclerosis, consistent with ABCA1's function in maintaining cholesterol homeostasis and HDL formation (5-8). ABCA1 is also a candidate risk gene for late onset Alzheimer's disease (LOAD), a neurodegenerative disease that is also linked to altered cholesterol transport and metabolism (9). ABCA1 dysfunction may contribute directly to Alzheimer's disease (AD) pathogenesis by accelerating amyloid-β (Aβ) production, which is one of the pathological hallmarks of AD (9).
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