Render Target: STATIC
Render Timestamp: 2024-11-21T13:20:28.966Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:41.844
Product last modified at: 2024-09-30T08:01:33.693Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ABCA7 (E7O5A) Rabbit mAb #32942

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 235
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ABCA7 (E7O5A) Rabbit mAb recognizes endogenous levels of ABCA7 protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu2149 of mouse ABCA7 protein.

    Background

    ATP-binding cassette (ABC) proteins are membrane-residing transporters that transport substrates across the membrane in an ATP-dependent manner. ABC substrates subject to active transport across the membrane include ions, amino acids, lipids, and sterols (1). ATP-binding cassette sub-family A member 7 (ABCA7) is a member of the ABC family and functions to regulate phospholipid and cholesterol homeostasis in central nervous system (CNS) as well as peripheral tissue. ABCA7, like most ABC transporters, contains two transmembrane domain bundles composed of six membrane-spanning helices and two nucleotide-binding domains. ABCA7 and its closest homolog, ABCA1, are 12A class members of ABCs and both proteins function to transport cholesterol and phospholipids in an apolipoprotein A (apoA) – dependent manner (2,3). ABCA7 is expressed in a variety of tissue and exhibits neuronal and microglial enrichment in the CNS (4). Human genetic studies identified ABCA7 gene variants, including loss-of-function mutations, that associate with late-onset Alzheimer’s disease (AD) (5). ABCA7 dysfunction may contribute directly to AD pathogenesis by accelerating amyloid-β (Aβ) production and/or altering microglia-dependent phagocytosis of the Aβ (4,6,7).
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