Render Target: STATIC
Render Timestamp: 2024-11-15T09:58:04.277Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:28:29.782
Product last modified at: 2024-05-30T07:07:23.266Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Abi3 Antibody #23060

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55, 65
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Abi3 Antibody recognizes endogenous levels of total Abi3 protein. This antibody recognizes a band at ~100 kDa of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu182 of human Abi3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Abi1, Abi2, and Abi3 are members of the Abl1 interactor family (Abi), which function as adaptor signaling molecules downstream of the receptor tyrosine kinase Abl1 (1-3). In addition to binding to Abl1, all Abi proteins contain a Src homology 3 (SH3) domain and interact with the WAVE complex, an actin regulator involved in membrane ruffling and lamellipodia formation (4,5). As cytoskeletal adaptor proteins, Abi proteins are likely to regulate actin cytoskeletal remodeling. Interestingly, ABI3, the gene encoding Abi3, is genetically linked to Alzheimer’s disease (AD). Abi3 may contribute to microglia-mediated AD progression by regulating microglial morphology (6,7).  
    For Research Use Only. Not For Use In Diagnostic Procedures.
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