ABIN-1 Antibody #4664
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80-85 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
ABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7). Overexpression of ABIN-1 inhibits NF-κB activation by a number of stimuli including TNF, IL-1, and LPS. It is widely expressed and can be induced by NF-κB, providing a negative feedback loop of NF-κB signaling. In addition to binding to A20, ABIN-1 can function on other key components of NF-κB signaling including IKKγ/NEMO (8) and NF-κB family members p100 and p105 (9), and can also inhibit ERK signaling (10). Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11). ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis.
- Verstrepen, L. et al. (2009) Biochem Pharmacol 78, 105-14.
- Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
- Lee, E.G. et al. (2000) Science 289, 2350-4.
- Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
- Wertz, I.E. et al. (2004) Nature 430, 694-9.
- Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82.
- Fukushi, M. et al. (1999) FEBS Lett 442, 83-8.
- Mauro, C. et al. (2006) J Biol Chem 281, 18482-8.
- Cohen, S. et al. (2009) Biochem Biophys Res Commun 389, 205-10.
- Zhang, S. et al. (2002) Biochem Biophys Res Commun 297, 17-23.
- Oshima, S. et al. (2009) Nature 457, 906-9.
Limited Uses
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