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Product last modified at: 2025-01-01T09:01:27.564Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ACE2 (E5O6J) XP® Rabbit mAb #92485

Filter:
  • WB
  • IHC
Western Blotting Image 1: ACE2 (E5O6J) XP®  Rabbit mAb
Western blot analysis of extracts from unmodified A549 cells (lane 1) and transgenic A549 cells stably expressing ACE2 protein (lane 2), using ACE2 (E5O6J) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Transgenic A549 cells were generously provided by Dr. Elena Piskounova, Weill Cornell Medicine.

To Purchase # 92485

Cat. # Size Qty. Price Ships
92485T 20 µl
$172
92485S 100 µl
$402

Supporting Data

REACTIVITY H M Hm
SENSITIVITY Endogenous
MW (kDa) 120-135
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Hm-Hamster 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:400 - 1:1600
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ACE2 (E5O6J) XP® Rabbit mAb recognizes endogenous levels of total ACE2 protein.

Species Reactivity:

Human, Mouse, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp201 of human ACE2 protein.

Background

ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy-terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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