AceCS2/ACSS1 (E6T5E) Rabbit mAb #37041

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	75
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AceCS2/ACSS1 (E6T5E) Rabbit mAb recognizes endogenous levels of total AceCS2/ACSS1 protein. This antibody does not cross-react with AceCS1/ACSS2 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly370 of human AceCS2/ACSS1 protein.

Background

Mitochondrial acetyl-CoA synthetase 2 (AceCS2), also known as acyl-CoA synthetase short-chain family member 1 (ACSS1), catalyzes acetyl-CoA biosynthesis from acetate and CoA. Studies show that acetate levels go up dramatically at sites of infection and inflammation, leading to the decrease of acetate assimilating enzymes AceCS2/ACSS1 and AceCS1/ACSS2 levels in memory CD8⁺ T cells. Acetate not metabolized by these enzymes instead stimulates glutaminase activity in memory CD8⁺ T cells, enhancing glutaminolysis, mitochondrial respiration, and cell survival (1). In addition, AceCS2/ACSS1 is highly expressed in one of the hepatocellular carcinoma (HCC) subtypes (2).

Database Links

UniProt ID: Q9NUB1

Entrez-Gene Id: 84532

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For Research Use Only. Not For Use In Diagnostic Procedures.

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