Name: Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb
Price: 327.00 USD
Availability: InStock

Filter:

WB
IP

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	280
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb recognizes endogenous levels of total acetyl-CoA carboxylase 2 protein and does not cross-react acetyl-CoA carboxylase 1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1400 of human acetyl-CoA carboxylase 2 protein.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

Database Links

UniProt ID: O00763

Entrez-Gene Id: 32

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578