Acetyl-Histone H2A.Z (Lys4/Lys7) (D3V1I) Rabbit mAb #75336
- WB
- IP
- F
- ChIP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 14 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Acetylation of Histone H2AZ correlates with gene activity (8). Acetylation of Histone H2AZ on Lys4 and Lys7 occurs at the 5' end of genes and confers nucleome destabilization and open chromatin confirmation required for tanscriptional activation (9-11).
- Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
- Raisner, R.M. and Madhani, H.D. (2006) Curr Opin Genet Dev 16, 119-24.
- Mizuguchi, G. et al. (2004) Science 303, 343-8.
- Kusch, T. et al. (2004) Science 306, 2084-7.
- Ruhl, D.D. et al. (2006) Biochemistry 45, 5671-7.
- Suto, R.K. et al. (2000) Nat Struct Biol 7, 1121-4.
- Fan, J.Y. et al. (2004) Mol Cell 16, 655-61.
- Millar, C.B. et al. (2006) Genes Dev 20, 711-22.
- Bruce, K. et al. (2005) Nucleic Acids Res 33, 5633-9.
- Ishibashi, T. et al. (2009) Biochemistry 48, 5007-17.
- Valdés-Mora, F. et al. (2012) Genome Res 22, 307-21.
Limited Uses
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