Acetyl-Histone H3 (Lys36) (D9T5Q) Rabbit mAb #27683
- WB
- IP
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Histone H3 Lys36 acetylation is mediated by the GCN5 histone acetyltransferase and is conserved in multiple species, from yeast to mammals (9). Acetyl-histone H3 Lys36 is localized predominantly to the promoters of active RNA polymerase II-transcribed genes and overlaps with other acetylation marks associated with transcriptional activation, such as acetyl-histone H3 Lys9 and Lys14 (9). The pattern of acetyl-histone H3 Lys36 is inversely related to that of tri-methyl-histone H3 Lys36, which is found in the gene body of actively transcribed genes.
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Roth, S.Y. et al. (2001) Annu Rev Biochem 70, 81-120.
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Yang, X.J. (2004) Bioessays 26, 1076-87.
- Haberland, M. et al. (2009) Nat Rev Genet 10, 32-42.
- Haigis, M.C. and Sinclair, D.A. (2010) Annu Rev Pathol 5, 253-95.
- Morris, S.A. et al. (2007) J Biol Chem 282, 7632-40.
Limited Uses
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