Render Target: STATIC
Render Timestamp: 2024-12-20T10:57:35.420Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:25:38.276
Product last modified at: 2024-12-09T23:15:08.562Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Acinus Antibody #4934

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 220, 84, 86
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Acinus Antibody detects endogenous levels of total Acinus protein. The antibody recognizes L, S and S' isoforms.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala835 of Acinus. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Acinus (apoptotic chromatin condensation inducer in the nucleus) is a caspase substrate that has been implicated in nuclear changes during apoptosis (1). Chromatin condensation and DNA fragmentation are both nuclear morphological features associated with apoptosis. Acinus is expressed in different isoforms (L, S, S') most likely generated by alternative splicing (1). During apoptosis Acinus is cleaved by caspase-3 to generate a 23 kDa fragment that was reported to induce chromatin condensation (1). Acinus has been identified to be a component of the spliceosome complex, ASAP, suggesting a role in pre-mRNA processing (2-4). Down regulation of Acinus by RNA interference inhibits cell growth (5). This study also found that loss of Acinus inhibits DNA fragmentation but not chromatin condensation during apoptosis.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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