Actin Nucleation and Polymerization Antibody Sampler Kit #8606
Product Information
Kit Usage Information
Protocols
- 2461: Western Blotting
- 3128: Western Blotting
- 3246: Western Blotting
- 3659: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 4651: Western Blotting
- 4738: Western Blotting
- 4848: Western Blotting, Immunoprecipitation (Agarose)
- 7074: Western Blotting
Product Description
Specificity / Sensitivity
Source / Purification
Activation state specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino termini of human rac1 and cdc42 proteins, the sequence of human ARP2 protein, or the amino terminus of human ARP3 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85.
- Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.
- Millard, T.H. et al. (2004) Biochem J 380, 1-17.
- Suetsugu, S. et al. (1999) Biochem Biophys Res Commun 260, 296-302.
- Goley, E.D. and Welch, M.D. (2006) Nat Rev Mol Cell Biol 7, 713-26.
- Witke, W. (2004) Trends Cell Biol 14, 461-9.
- Boukhelifa, M. et al. (2006) FEBS J 273, 26-33.
- Gareus, R. et al. (2006) J Biol Chem 281, 2803-11.
- Reinhard, M. et al. (1995) EMBO J 14, 1583-9.
- Suetsugu, S. et al. (1998) EMBO J 17, 6516-26.
Limited Uses
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