Render Target: STATIC
Render Timestamp: 2024-12-26T12:06:57.169Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:22:31.169
Product last modified at: 2024-05-30T07:13:03.527Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ADAP Antibody #11957

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 120, 130
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ADAP Antibody recognizes endogenous levels of total ADAP protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human ADAP protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    ADAP (adhesion and degranulation-promoting adaptor protein/SLAP-130/Fyb) is an SH3 domain-containing adaptor protein expressed by T cells, NK cells, and myeloid cells (1,2). There are two isoforms of ADAP with predicted molecular weights of 85 kDa and 90 kDa, but observed molecular weights of 120 kDa and 130 kDa (1-3). ADAP was identified as an adaptor protein that interacts with SLP-76 following T cell receptor (TCR) stimulation and was subsequently found to be important for several aspects of T cell activation (1,2). For example, ADAP is required for integrin-dependent clustering, signaling, and adhesion (4,5). In addition, ADAP interacts with CARMA1 and facilitates assembly of the CARMA1-Bcl10-MALT1 complex important for NF-κB activation downstream of TCR activation (6). Finally, following binding of a T cell to an antigen presenting cell, ADAP forms a ring at the immunological synapse that recruits dynein to enable microtubule-organizing center polarization (7).
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