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Render Timestamp: 2025-01-22T02:08:50.518Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:59:58.119
Product last modified at: 2025-01-15T20:30:10.519Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ADK (F5Q5P) Rabbit mAb #93994

Filter:
  • WB
  • IP
Western Blotting Image 1: ADK (F5Q5P) Rabbit mAb
Western blot analysis of extracts from various cell lines using ADK (F5Q5P) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of ADK protein in SK-MEL-28 and OV-90 cells is consistent with the predicted expression pattern.

To Purchase # 93994

Cat. #

Size

93994T
20 µl
93994S
100 µl

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 45
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ADK (F5Q5P) Rabbit mAb recognizes endogenous levels of total ADK protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ADK protein.

Background

Adenosine kinase (ADK) is an evolutionary conserved ribokinase that functions as a key regulator of adenosine metabolism by catalyzing the phosphorylation of adenosine to produce 5-adenosine monophosphate (AMP) (reviewed in 1). Adenosine is not only a critical component of RNA, but also functions in maintaining energy homeostasis as a precursor of ATP production, second messenger signaling through the production of cAMP, maintaining S-adenosylmethionine-dependent transmethylation and adenine-containing co-enzymes NAD and FAD, and can directly signal through its cognate receptors. ADK has two isoforms with distinct expression and subcellular localization: a nuclear long form (ADK-L) and a cytoplasmic short form (ADK-S) (2). Genetic knockout of ADK in mice results in perinatal lethality, microvesicular hepatic steatosis, and stunted growth (3). ADK dysfunction is involved in several pathologies, including diabetes, epilepsy, and cancer (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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