Render Target: STATIC
Render Timestamp: 2024-12-26T12:11:23.220Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:54.168
Product last modified at: 2024-11-14T17:00:14.848Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit mAb #27987

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 30-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit mAb recognizes endogenous levels of total mouse Adora2b/Adenosine Receptor A2b protein. This antibody cross-reacts with human Adenosine receptor A2a protein but not with mouse Adenosine receptor A2a protein or human Adenosine receptor A2b protein. This antibody detects a 140 kDa band of unknown origin, and in human cell lines, detects a 42 kDa band of unknown origin.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Adora2b/Adenosine Receptor A2b protein.

    Background

    Adenosine receptor A2b (A2B adenosine receptor, A2BAR, ADORA2B) belongs to the adenosine receptor group of G protein-coupled receptors (GPCRs) (1-6). The adenosine receptors are divided into A1, A2, and A3 subtypes based on their ability to inhibit or stimulate adenyl cyclase (1-3). Within the A2 subtype, the high-affinity receptor is designated the A2A adenosine receptor, and the low-affinity receptor is the A2B adenosine receptor (1-3). Adenosine receptor A2b has been identified as a regulator of inflammation and vascular adhesion, primarily via signals from hematopoietic cells to the vasculature (3). Adenosine receptor A2b couples to G proteins to activate adenyl cyclase, leading to increased intracellular cAMP levels, as well as activation of phospholipase C (PLC), leading to increased intracellular calcium (4-6). Multiple mediators associated with inflammation, such as LPS, TNF-α, and IFN-γ, have been demonstrated to increase A2BAR expression in certain cell types (4-6).

    Database Links

    UniProt ID: P29275


    Entrez-Gene Id: 136


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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