AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated) #20112
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human AE1 protein.
Background
Anion exchange protein 1 (AE1), also named solute carrier family 4 member 1 (SLC4A1), is an anion transporter that mediates chloride-bicarbonate exchange in the kidney and regulates normal acidification of the urine (1,2). A different isoform of AE1 is a major integral membrane structure protein of erythrocytes, where it plays a critical role in the removal of carbon dioxide from tissues (3). In addition, AE1 is required for normal flexibility and stability of the erythrocyte membrane. Mutations in SLC4A1 can lead to hereditary spherocytosis, ovalocytosis, and distal renal tubular-acidosis (4-7). Other mutations that do not cause disease became novel blood group antigens, which are part of the Diego blood group system (8).
- Kopito, R.R. and Lodish, H.F. Nature 316, 234-8.
- Kollert-Jöns, A. et al. (1993) Am J Physiol 265, F813-21.
- Swenson, E.R. et al. (1993) J Appl Physiol (1985) 74, 838-48.
- Jarolim, P. et al. (1991) Proc Natl Acad Sci U S A 88, 11022-6.
- Maillet, P. et al. (1995) Br J Haematol 91, 804-10.
- Quilty, J.A. et al. (2002) Am J Physiol Renal Physiol 282, F810-20.
- Escobar, L. et al. (2013) Nefrologia 33, 289-96.
- Figueroa, D. (2013) Immunohematology 29, 73-81.
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