Render Target: STATIC
Render Timestamp: 2024-12-23T10:43:21.244Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-12 00:05:10.658
Product last modified at: 2024-12-12T14:45:10.984Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

AFP (C3) Mouse mAb #39971

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG2a
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:50 - 1:200
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AFP (C3) Mouse mAb recognizes endogenous levels of alpha-fetoprotein (AFP).

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with serum of a hepatoma patient that has affinity isolated human alpha-fetoprotein (AFP).

    Background

    Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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