AKAP5 (D28G3) Rabbit mAb #5671
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 79 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg383 of human AKAP5 protein.
Background
AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell (1). Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RIα, RIIα, RIβ, or RIIβ), individual AKAPs contain additional domains responsible for the recruitment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinases, etc.) or restricting AKAP to a specific subcellular location (1). AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities (2-4). AKAP5 specifically binds to the regulatory subunit of PKAIIβ, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions (4-5). The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types (5-8).
- Schwartz, J.H. (2001) Proc Natl Acad Sci U S A 98, 13482-4.
- Bregman, D.B. et al. (1991) J Biol Chem 266, 7207-13.
- Hirsch, A.H. et al. (1992) J Biol Chem 267, 2131-4.
- Carr, D.W. et al. (1992) J Biol Chem 267, 16816-23.
- Glantz, S.B. et al. (1993) J Biol Chem 268, 12796-804.
- Klauck, T.M. et al. (1996) Science 271, 1589-92.
- Sarkar, D. et al. (1984) J Biol Chem 259, 9840-6.
- Coghlan, V.M. et al. (1995) Science 267, 108-11.
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