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ALDH1A2 Antibody #83805

Filter:
  • WB
  • IP
Western blot analysis of extracts from various cell lines using ALDH1A2 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The relative expression of ALDH1A2 among cell lines was confirmed by mass spectrometry.

To Purchase # 83805

Cat. # Size Qty. Price
83805S 100 µl
$306

Product Specifications

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 55
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ALDH1A2 Antibody recognizes endogenous levels of total ALDH1A2 protein. Based on sequence comparisons, this antibody is not predicted to cross-react with ALDH1A1.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly350 of human ALDH1A2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6).
For Research Use Only. Not for Use in Diagnostic Procedures.
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