Render Target: STATIC
Render Timestamp: 2024-11-21T13:28:19.369Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:03.056
Product last modified at: 2024-10-31T17:45:09.265Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ALOX12 (E3O9P) Rabbit mAb #49874

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 76
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALOX12 (E309P) Rabbit mAb recognizes endogenous levels of total ALOX12 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly608 of human ALOX12 protein.

    Background

    Lipoxygenases catalyze the oxidation of polyunsaturated fatty acids and have a variety of physiological functions as well as roles in disease (1). ALOX12 (arachidonic acid 12-lipoxygenase) is an important lipoxygenase that specifically converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid (12- HpETE), which is then reduced by glutathione peroxidase to 12-hydroxyeicosatetraenoic acid (12-HETE). ALOX12 plays a role in regulating platelet aggregation, cell migration, and proliferation and is involved in diseases, such as thrombosis, atherosclerosis, diabetes, neurodegeneration, inflammatory disease, and cancer (2). ALOX12 is highly expressed in nonalcoholic steatohepatitis (NASH) and can regulate disease progression by interacting with acetyl-CoA carboxylase 1 (ACC1) (3,4). ALOX12 is also upregulated during hepatic ischemia leading to inflammation and cell death (5). ALOX12 inhibition also abrogates p53-mediated ferroptosis and tumor suppression but does not affect other ferroptosis pathways such as that driven by GPX4 (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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