Androgen Receptor Antibody Sampler Kit #61949
Product Information
Kit Usage Information
Protocols
- 5153: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
- 7074: Western Blotting
- 19672: Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
- 70317: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
Product Description
Specificity / Sensitivity
Source / Purification
Background
The AR3 or AR-V7 isoform, which lacks the typical ligand binding domain, is created through the alternative splicing of cryptic exons (4-5). AR-V7 is frequently expressed in castration-resistant prostate cancer (CRPC) and while dependent on the activity of the full-length androgen receptor (AR-FL), AR-V7 can activate a completely distinct transcriptional program (6-8). While enzalutamide and abiraterone have been beneficial in treating CRPC through the ligand binding domain of AR-FL, resistance in patients has been shown to be associated with AR-V7 detection in circulating tumor cells (9-12). Studies probing into mechanisms of overcoming this resistance are currently being explored and may help in stratifying patient populations for more personalized therapies (13-15).
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Limited Uses
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