Androgen Receptor (E3S4N) Rabbit mAb (Carboxy-terminal Antigen) (ChIP Formulated) #82055

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ChIP

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

Application	Dilution
Chromatin IP	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Androgen Receptor (E3S4N) Rabbit mAb (Carboxy-terminal Antigen) (ChIP Formulated) recognizes endogenous levels of total Androgen Receptor protein. This antibody does not detect AR-V7 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val662 of human Androgen Receptor protein.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).

Database Links

UniProt ID: P10275

Entrez-Gene Id: 367

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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