Render Target: STATIC
Render Timestamp: 2025-01-22T10:14:10.272Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:58:04.130
Product last modified at: 2025-01-01T09:03:07.157Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Angiomotin (E3E7X) Rabbit mAb #51142

Filter:
  • WB
Western Blotting Image 1: Angiomotin (E3E7X) Rabbit mAb
Western blot analysis of extracts from 293T and MCF7 cells using Angiomotin (E3E7X) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of angiomotin protein in MCF7 cells is consistent with the predicted expression pattern.

To Purchase # 51142

Cat. # Size Qty. Price Ships
51142T 20 µl
$153
51142S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 140, 130, 100
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Angiomotin (E3E7X) Rabbit mAb recognizes endogenous levels of angiomotin isoform 1 (full-length) protein. This antibody does not recognize the amino-terminally truncated angiomotin isoform 2 protein. This antibody cross-reacts with the angiomotin family members, angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2).

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human angiomotin protein.

Background

Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.