Annexin A6 Antibody #29015
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 72 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Annexin A6 Antibody recognizes endogenous levels of total annexin A6 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr645 of human annexin A6 protein. Antibodies are purified by peptide affinity chromatography.
Background
Annexin A6 (ANXA6) belongs to the highly conserved annexin family of proteins characterized by their calcium (Ca2+)-dependent binding to phospholipids (1-3). The protein is highly expressed in most tissues and is predominantly located at the plasma membrane and the endosomal compartment, where it regulates cell migration and endosome trafficking by organizing membrane domains, interacting with signaling complexes, and forming transient membrane-actin interactions upon Ca2+ stimulation (3). It also mediates intracellular cholesterol homeostasis (3,4). Annexin A6 is frequently upregulated in extracellular vesicles (EVs) of the tumor microenvironment after chemotherapy or tyrosine kinase inhibitor treatment. These EVs have been shown to promote resistance and metastasis in multiple cancer types, such as lung cancer, breast cancer, and pancreatic duct adenocarcinoma (5-7). Annexin A6, in these EVs, enhances the stability of membrane-associated RTKs, such as EGFR, and promotes cancer cell motility, stemness, and autophagy (2,7-9).
- Gerke, V. and Moss, S.E. (2002) Physiol Rev 82, 331-71.
- Korolkova, O.Y. et al. (2020) Cells 9, 1855. doi: 10.3390/cells9081855.
- Grewal, T. et al. (2017) Cell Adh Migr 11, 288-304.
- Enrich, C. et al. (2011) Biochim Biophys Acta 1813, 935-47.
- Keklikoglou, I. et al. (2019) Nat Cell Biol 21, 190-202.
- Leca, J. et al. (2016) J Clin Invest 126, 4140-4156.
- Li, T. et al. (2021) Cell Death Dis 12, 684.
- Sakwe, A.M. et al. (2011) Exp Cell Res 317, 823-37.
- Guo, Z. et al. (2021) Front Cell Dev Biol 9, 718721.
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